According to the concept of our research group, erythrocytes value the microcirculation and homorheologic condition, so called homorhelogical status.

We were interested in how the hemorheological status has been changing in adults during the dysfunction of thyroid, the second stage of periodontitis and the parallel development of the thyroid gland and periodontitis. If we take into consideration that according to the epidemiplogical data in children and adults throughout the world the thyroid disease is spreading and increasing from 10 to 15 years of age, the changes in characteristic of periodontitis is far more common in children [15,16].

Our research is very important for fundamental research as well as for practical biomedicine. This approach is gaining more relevance in research, as it is for the first time that we are connecting these two pathologies and estimate rheological status.

Materials and Methods. We investigated 75 patients (30 boys and 45 girls) 11 – 15 years old. Group I – patients with thyroid dysfunction, n=25,  maen age =11,9±2,0   without treatment; group II – patients with II stage periodontitis, n=25, mean age = 12,5±1,6 with out treatment; group III – patients with nozological, wheare parallel development of hyroid dysfunction and periodontitis n=25, mean age = 12,8±1,3 with out treatment; group IV – control subjects group, n=10, mean age = 12,4±2,0.

The following rheological status were examined erythrocytes aggregability, which represents aggregated erythrocytes area ratio against whole area of the erythrocytes [7]. Erythrocyte aggregation was evaluated with the recently developed “Georgian technique” [8,10] providing us with direct and quantitative data. Blood samples (4ml) from the cubital veins were centrifuged and about 0.1 ml blood was diluted 1:200 in own plasma in the Thoma pipettes preliminary rinsed with 5% sodium citrate solution without addition of any other anticoagulants to the blood under study. Following standard mixing the diluted blood was placed into a glass chamber 0.1 mm high. The quantitative index of erythrocyte aggregation, which was assessed with a special program at the Texture Analysis System (TAS-plus, “Leitz, Germany), represented itself the relationship of the aggregated and unaggregated red cells.

Data are presented as mean ±SD. Comparison of data were evaluated by Student’s paired t-test, while data from the patients’ study were evaluated by Student’s unpaired t-test. Differences between groups were considered statistically if P<0.001.

Studies conducted charges according the Declaration of Helsinki [14].

Results. Rheologiocal status in Group I 30,0±2,5. Rheological status in Group II 25,8±2,6. Rheologiocal status in Group III 32,2±2,0. Rheologiocal status in control  – 22,4±2,0.

Discussion. According to the obtained results it turned out that during periodontitis the rheological features are disordered compared to the control (status deteriorated by 15%). The rheological features are far more disordered during dysfunction of thyroid (status deteriorated by 30%). The children who had periodontitis and the dysfunction of thyroid at the same time have been examined. In the given case the rheological status was far from the normal rheological situation for 40% (the targets of our research were the primary patients who weren’t provided with hormonal treatment).

From our point of view periodontitis and the dysfunction of the thyroid developed in parallel. It is known that without an adequate blood supply the normal functioning of the tissue and organ is impossible. The provision of the abovementioned is made by the normal flow of micro circular network, which itself depends on the rheological features of the blood. Micro circulation is important during each physiological process and far more important during the development of pathological processes. Pathology causes the disorder of micro circulation, hemorhelogical changes and the latter strengthens the pathological processes [8,9].

Thus we can conclude that thyroid disorders are contributing factors to the development of periodontitis. Despite the lack of works [1,4,5], where hemorhelogical, the dysfunction unity of calcium and phosphorus homeostasis gland, are discussed, our data also confirms the truth of this statement. We believe that the microcirculation disorder is caused by the mineralization imbalance as a result of parathormonis not only locally, but also in every organ and tissue. The development of periodontitis, cardiovascular problems and risks, as well as memory and other neuro- and neurogenic risk factors are derived from the above mentioned [1,2,3].

Local trans capillary metabolism cause hemorhelogical changes. On one hand hemorhelogical disorders cause illness and on the other hand microcirculation disorder develops pathologies. The more microcirculation is damaged the more actively the rheological status is changed and the more heavily organs and tissues are damaged, which in turn do not function normally and even more damage the microcirculation network by excess or small amounts of a hormone excretion or exudation of other substances [6].

The issue is very relevant, having a practical applied side. It was concluded that one of the reasons of the development of periodontitis in the adults is the changes in the functional state of the thyroid.

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10.    Mchedlishvili, G., Beritashvili N., Lominadze D., and Tsinamdzvrishvili B.  Technique for Direct and Quantitative-Evaluation of Erythrocyte Aggregability. J. Biorheology. 1993. 30, p.153–161.

11.    Nikolaishvili M., Beriashvili S., Franchuk K., Tupinashvili T., Vashakidze I., Zenaishvili S. Crystallization of a saliva at teenagers at dysfunction of a thyroid gland. J. Experimental and clinical Medicine. 2014. 4, p.29-33.

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14.    https://www.ub.edu/recerca/Bioetica/doc/Declaracio_Helsinki_2013.pdf.

15.     https://www.who.int/entity/bulletin/volumes/90/8/12-040812.